PhenoSys Mini2P

The Mini2P is a miniature two-photon microscope for fast, high-resolution, multi-plane calcium imaging in freely moving mice. Weighing under 3 g with a highly flexible connection cable, Mini2P enables stable imaging without hindering animal behavior. Its optimized optical system allows stable simultaneous recordings of neuronal activity of more than a thousand cells in different brain regions.

Features

Overview

Ultra-light microscope body (2.4g) with MEMS scanner for X-Y-scanning and µTlens for Z-scanning.
Two photon laser (920nm) with clean femtosecond pulses (<100 fs).
Light and flexible fiber optics and cable, all at a length of 2.2 m for maximal freedom to move for the animal.
Detection unit with two high-sensitive GaAsP-type photomultipliers.
DAQ-BNC interface board for flexible integration/synchronization of/with additional devices.
DAQ computer with preinstalled and configured Matlab-based software (ScanImage).
Compact hardware design, everything integrated on a mobile trolley.
The package comes ready-to-use.
Full technical support
Fast imaging (15 Hz for 256×256 pixels) in a large field of view 500×500 µm2.
Multilayer imaging into 240 µm depth.
Maximum resolution of 1.3 µm x 1.3 µm x 15 µm.
Set of interchangeable objectives to image e.g. visual cortex, medial entorhinal cortex or hippocampus.
Simultaneous activity imaging of more than 1,000 neurons in one region or recording of more than 10,000 cells in adjacent field-of-views.
Stable high-quality imaging across a wide spectrum of mouse behaviors, including instances such as climbing and jumping.
Raw data is fully accessible and the system is individually extendable.

Your Partner in Research

As a licensed PhenoSys supplier, we guarantee expert support from a trusted US-based team to ensure your research needs are met.

Specs

Spec
Single wavelength fiber laser:	920 nm, <100 fsec
Mini2P microscopy body:
Weight:	2.4g
	Quartet of microtunable lenses (µTlens) for z-scanning with 240 µm depth
	MEMS mirror with 15 Hz frame rate at 256 x 256 pixels for a field of view of 500 µm x 500 µm
Resolution:	1.3 µm x 1.3 µm x 15 µm
	Base plate and stitching adapter (for FOVs of up to 4 mm2)
Interchangeable objectives:
	D0213 (with cover glass to image e.g. visual cortex)
	D0254 (with GRIN lens to image e.g. hippocampal CA1)
	D0277 (with prism to image e.g. medial entorhinal cortex)
Excitation fiber:	Length: 2.2 m
Collection fiber:	Length: 2.5 m
Cable Harness:
	6-wire bundle to control µTlens and MEMS mirror
	Length: 2.5 m

PhenoSys Mini2P

Features

Overview

Your Partner in Research

Specs

Gallery

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